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THE SCIENCE BEHIND

LAB QUALITY HEALTH ANALYSIS AT HOME
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Daisy is able to detect the very early stages of caries and gum disease thanks to the scientific technology that supports its purpose.

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Our innovation is based on certified lab tests that are able to process your saliva sample giving you high accuracy, fast processing results.

 

Daisy's soul is the Elisa Test, an enzymatic test that through a reaction between specific antibodies solvents, selected for the identification of the pathologies, and the enzyme Metalloproteinase 8 (MMP8) can process the microbiological components and releases fast, accurate lab results to you, directly at your home.

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AT THE CORE OF OUR INNOVATION

Our goal is to do effective and real prevention.

How to reach this purpose?

Identifying the most common oral issues at their source, before their visible presence in the mouth.

We researched a way to collect meaningful information related to the biological composition of caries and periodontitis.

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We discover speaking with experts and microbiologists that thanks to saliva you can be able to receive a universe of different information related to your general health statusAre you curious about it?

Continue to scroll and you'll see

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It's really an incredible substance that can be a map of our health, let's take a look at how many information she can give us!

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Among the many data regarding body health, we focus in those related to the oral condition.

So we started researching in the saliva composition the specific elements that we needed for detecting the oral issues interested.

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With the support of the experts, that collaborate with us in the lab, we found the relevant pathology biomarkers to study and we discover a method to elaborate them in order to process a saliva sample for obtaining the result about the oral condition!   

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With the support of the experts, that collaborate with us in the lab, we found the relevant pathology biomarkers to study and we discover a method to elaborate them in order to process a saliva sample for obtaining the result about the oral condition!   

What is the functional system of the test?

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As we said previously the core of our innovation is the Elisa Test (= Enzyme-Linked ImmunoSorbent Assay) is an immunoassay technique that quantifies the concentrations of various molecules present in biological fluids.

Synthetically is a lab test that, linking antibodies with a specific antigen (MMP8), is able to detect the quantitative presence of the biological element that interests us, the metalloproteinase 8.

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But we studied a particular version on this enzymatic test: the Elisa Sandwich developed with two different antibodies that can detect the different characteristics and actors related to each oral disease.

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How do the processes work?

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There is a common starting point in the recognition of these oral pathologies.

Everything starts from a particular fluid that comes up from the little channels inside the tooth, under the enamel.

It is called the crevicular fluid.

When it comes up in the mouth it brings with him many different microbiological elements, then as much information, and it flows in our saliva.

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And it's here that our test takes the stage!

The element that flows in the saliva that we need to detect is the MMP8 enzyme and we can analyze it with our tests for both pathologies because it's involved in each of them! 

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A short story about caries disease.

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When you have caries it happens because your teeth enamel is being damaged by different pathogenic microorganisms present in the oral cavity, mainly those attached to your teeth in the form of bacterial plaque.

This one is an aggregate (biofilm) of germs tenaciously attached to each other and to dental surfaces, which promotes and supports caries and gum disease.

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How our enzyme test can help you to prevent this common oral problem?

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It can detect the quantity of plaque-forming microorganisms since the very early moments of their presence. 

Through this test, we can warn you if the pathogen bacterial microorganism level is growing up and you will be able to prevent caries with a simple hygiene session to your dentist!

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A short story about gum disease

When the gums are in danger it's because the enzymes are damaging the collagen tissue supporting the teeth and it's for this that, in the advanced stage of pathologies, you risk losing your teeth!

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How our enzyme test can help you to prevent this common oral problem?

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It can detect the quantity of enzymes since the very early stage of this oral issue. Through this test, we can warn you if the enzyme level is growing up and you will be able to prevent the gum disease thank your dentist action!

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transparent science

1) Sapna G, Gokul S, Bagri-Manjrekar K. Matrix metalloproteinases and periodontal diseases, Oral Dis. 2014 Sep;20(6):538-550 (https://pubmed.ncbi.nlm.nih.gov/23849049/);

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2)T. Sorsa, M. Hernández, J. Leppilahti, S. Munjal, L. Netuschil, and P. Mäntylä, Detection of gingival crevicular fluid MMP-8 levels with different laboratory and chair-side methods, Oral Diseases, vol. 16, no. 1, pp. 39-45, 2010, (https://researchportal.helsinki.fi/en/publications/detection-of-gingival-crevicular-fluid-mmp-8-levels-with-differen);

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3) M E Ryan 1S RamamurthyL M Golub. Matrix Metalloproteinases and Their Inhibition in Periodontal Treatment, Curr Opin Periodonto1996;3:85-96 (https://pubmed.ncbi.nlm.nih.gov/23849049/);

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4)Karolina E Kaczor-Urbanowicz, Harsh M Trivedi, Patricia O Lima, Paulo M Camargo, William V Giannobile, Tristan R Grogan, Frederico O Gleber-Netto, Yair Whiteman, Feng Li, Hyo Jung Lee, Karan Dharia, Katri Aro, Carmen Martin Carreras-Presas, Saarah Amuthan, Manjiri Vartak, David Akin, Hiba Al-Adbullah, Kanika Bembey, Perry R Klokkevold, David Elashoff, Virginia M Barnes, Rose Richter, William DeVizio, James G Masters, David T W Wong, Salivary exRNA Biomarkers to Detect Gingivitis and Monitor Disease Regression, J Clin Periodontol 2018 Jul;45(7):806-817, (https://pubmed.ncbi.nlm.nih.gov/29779262/);

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5) Atul Jaina, Rachana Bahugunab, Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview​J Oral Biol Craniofac Res. 2015 Sep-Dec; 5(3): 212–218, (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623218/);

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6) Jana Schmidt, Markus Kreuz, Wieland Kiess, Dirk Ziebolz, Ulrike Guder, Markus Löffler, Christian Hirsch, Rainer Haak, AMMP-8 in correlation to caries and periodontal condition in adolescents-results of the epidemiologic LIFE child study, Clinical oral investigation ER 10.1007/s00784-017-2132-0
JO (
https://www.researchgate.net/publication/317348889_AMMP-8_in_correlation_to_caries_and_periodontal_condition_in_adolescents-results_of_the_epidemiologic_LIFE_child_study);

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7) Anders Hedenbjörk-Lager, Lars Bjørndal, Anders Gustafsson, Timo Sorsa, Leo Tjäderhane, Sigvard Ã…kerman, Dan Ericson, Caries Correlates Strongly to Salivary Levels of Matrix metalloproteinase-8​, Caries Res
. 2015;49(1):1-8. doi: 10.1159/000360625. Epub 2014 Jul 31, (https://pubmed.ncbi.nlm.nih.gov/25096527/);

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8)Chen-Zi Zhang, Xing-Qun Cheng, Ji-Yao Li, Ping Zhang, Ping Yi,  Xin Xu, Xue-Dong Zhou, Saliva in the diagnosis of diseasesInt J Oral Sci. 2016 Sep; 8(3): 133–137, (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5113094/)

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9) John Emmanwel Aljhu C. Del Rosario, Analysis of SalivaDepartment of Biology, College of Science, PUP Sta. Mesa, Manila (https://www.academia.edu/9658009/Analysis_of_Saliva);

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10) Raymond G. Schipper, Erika Silletti, Monique H. Vingerhoeds, Saliva as research material: Biochemical, physicochemical and practical aspectsj.archoralbio.2007 June 09, Article history: accepted 23 June 2007, (https://www.academia.edu/36684909/Saliva_as_research_material_Biochemical_physicochemical_and_practical_aspects);

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thank you!

From the team, we are grateful for the experts and the microbiologists that are helping and supporting us in our goal: to bring Daisy into reality!

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In particular, we would like to thank the University Dental Clinic in Bologna, with the precious help of Dr. Montevecchi, that had the patience to explain and show us the microbiological process involved in the test and made us fell in love with this interesting field. 

 

Thanks to Veronica Rivi, a biologist from the University of Modena and Reggio Emilia, for helping us through the Elisa Test world and encouraging our dream with enthusiasm.

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Also, the help of Giada Giulia Riso, clinical research coordinator from the S. Orsola hospital in Bologna, is very precious in our challenge for the study of the chemical processes involved in the test procedure and the research of the scientific articles related.

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Special thanks to Medicover Stomatologia Lab forn us through the research in the clinical field!

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